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Development of Double Antibody Sandwich ELISA for Detection of Duck or Goose Flavivirus

dc.contributor.authorHui-min NIU
dc.contributor.authorXin-mei HUANG
dc.contributor.authorKai-kai HAN
dc.contributor.authorYu-zhuo LIU
dc.contributor.authorDong-min ZHAO
dc.contributor.authorJing-feng ZHANG
dc.contributor.authorFei LIU
dc.contributor.authorTong-tong LI
dc.contributor.authorXiao-bo ZHOU
dc.contributor.authorXiang-rui LI
dc.contributor.authorYin LI
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China; NIU Hui-min, Correspondence LI Yin, Tel: +86-25-84391687, Fax: +86-25-84390330
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China; College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China
dc.contributor.otherCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R. China
dc.contributor.otherInstitute of Veterinary Medicine, Jiangsu Academy of Agricutural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-Products, Nanjing 210014, P.R. China; NIU Hui-min, Correspondence LI Yin, Tel: +86-25-84391687, Fax: +86-25-84390330
dc.date.accessioned2025-10-09T05:25:56Z
dc.date.available2025-10-09T05:25:56Z
dc.date.issued01-09-2013
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S2095311913603329
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/41006
dc.description.abstractIn order to establish double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of duck or goose flavivirus, polyclonal antibody against the flavivirus strain JS804 in geese and monoclonal antibody against the E protein of flavivirus strain JS804 in geese were used as the capture antibody and detection antibody, respectively. The optimal dilution of the capture antibody and detecting antibody capable of detecting the flavivirus strain JS804 in geese were 1:3 200 and 1:160 in the check-board titration, respectively. The reaction time of sample was 1 h, and the optimal working dilution of HRP-labeled goat-anti-mouse IgG was 1:10 000. The positive standard value was 0.247 (OD450 nm). The geese flavivirus could be detected at a minimal concentration of 1.875 μg mL−1. The ELISA had no cross-reaction with Newcastle disease virus (NDV), Avian influenza virus (AIV), Infectious bronchitis virus (IBV), Infectious bursal disease virus (IBDV), Duck hepatitis virus (DHV), and Gosling plague virus (GPV). Twenty clinical samples were detected by the DAS-ELISA and RT-PCR respectively, with the agreement rate of 75%. The results revealed that the DAS-ELISA possessed favorable specificity and higher sensitivity, indicating a suitable method for rapid detection of the duck or goose flavivirus.
dc.language.isoEN
dc.publisherElsevier
dc.subject.lccAgriculture (General)
dc.titleDevelopment of Double Antibody Sandwich ELISA for Detection of Duck or Goose Flavivirus
dc.typeArticle
dc.description.keywordsgoose
dc.description.keywordsflavivirus
dc.description.keywordsdouble antibody sandwich ELISA
dc.description.keywordsmonoclonal antibodies
dc.description.pages1638-1643
dc.description.doi10.1016/S2095-3119(13)60332-9
dc.title.journalJournal of Integrative Agriculture
dc.identifier.oaioai:doaj.org/journal:1d584c9679ea44aa951070b4de22604a
dc.journal.infoVolume 12, Issue 9


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