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Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis

dc.contributor.authorHedwig S. Kruitwagen
dc.contributor.authorLoes A. Oosterhoff
dc.contributor.authorIngrid G.W.H. Vernooij
dc.contributor.authorIngrid M. Schrall
dc.contributor.authorMonique E. van Wolferen
dc.contributor.authorFarah Bannink
dc.contributor.authorCamille Roesch
dc.contributor.authorLisa van Uden
dc.contributor.authorMartijn R. Molenaar
dc.contributor.authorJ. Bernd Helms
dc.contributor.authorGuy C.M. Grinwis
dc.contributor.authorMonique M.A. Verstegen
dc.contributor.authorLuc J.W. van der Laan
dc.contributor.authorMeritxell Huch
dc.contributor.authorNiels Geijsen
dc.contributor.authorRobert G. Vries
dc.contributor.authorHans Clevers
dc.contributor.authorJan Rothuizen
dc.contributor.authorBaukje A. Schotanus
dc.contributor.authorLouis C. Penning
dc.contributor.authorBart Spee
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands; Corresponding author
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine & Institute of Biomembranes, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Biochemistry and Cell Biology, Faculty of Veterinary Medicine & Institute of Biomembranes, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands
dc.contributor.otherDepartment of Surgery, Erasmus MC-University Medical Center, 3000 CA Rotterdam, the Netherlands
dc.contributor.otherDepartment of Surgery, Erasmus MC-University Medical Center, 3000 CA Rotterdam, the Netherlands
dc.contributor.otherHubrecht Institute, University Medical Centre, Utrecht University, 3584 CT Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands; Hubrecht Institute, University Medical Centre, Utrecht University, 3584 CT Utrecht, the Netherlands
dc.contributor.otherHubrecht Institute, University Medical Centre, Utrecht University, 3584 CT Utrecht, the Netherlands
dc.contributor.otherHubrecht Institute, University Medical Centre, Utrecht University, 3584 CT Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.contributor.otherDepartment of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, the Netherlands
dc.date.accessioned2025-10-09T05:29:45Z
dc.date.available2025-10-09T05:29:45Z
dc.date.issued01-04-2017
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S2213671117300826
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/41086
dc.description.abstractSummary: Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the potential of liver organoids for modeling genetic diseases. To examine the possibility of using organoids to model steatosis, we established a long-term feline liver organoid culture with adult liver stem cell characteristics and differentiation potential toward hepatocyte-like cells. Next, organoids from mouse, human, dog, and cat liver were provided with fatty acids. Lipid accumulation was observed in all organoids and interestingly, feline liver organoids accumulated more lipid droplets than human organoids. Finally, we demonstrate effects of interference with β-oxidation on lipid accumulation in feline liver organoids. In conclusion, feline liver organoids can be successfully cultured and display a predisposition for lipid accumulation, making them an interesting model in hepatic steatosis research. : In this study Kruitwagen and colleagues establish and characterize a feline liver organoid culture, which has adult stem cell properties and can be differentiated toward hepatocyte-like cells. They propose liver organoids as a tool to model hepatic steatosis and show that feline liver organoids accumulate more lipids than human organoids when provided with excess fatty acids. Keywords: feline liver organoids, adult liver stem cells, hepatic steatosis, disease modeling, feline hepatic lipidosis, species differences
dc.language.isoEN
dc.publisherElsevier
dc.subject.lccMedicine (General)
dc.titleLong-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis
dc.typeArticle
dc.description.pages822-830
dc.description.doi10.1016/j.stemcr.2017.02.015
dc.title.journalStem Cell Reports
dc.identifier.oaioai:doaj.org/journal:1b75311bbf3843328b88a26d9590e37a
dc.journal.infoVolume 8, Issue 4


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