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Utilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality

dc.contributor.authorQiqing Shen
dc.contributor.authorHaihua Ruan
dc.contributor.authorHongyang Zhang
dc.contributor.authorTao Wu
dc.contributor.authorKexin Zhu
dc.contributor.authorWenying Han
dc.contributor.authorRui Dong
dc.contributor.authorTianwei Ming
dc.contributor.authorHaikun Qi
dc.contributor.authorYan Zhang
dc.date.accessioned2025-10-09T05:30:45Z
dc.date.available2025-10-09T05:30:45Z
dc.date.issued01-03-2024
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2024.1375120/full
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/41108
dc.description.abstractFilamentous fungi play a crucial role in environmental pollution control, protein secretion, and the production of active secondary metabolites. The evolution of gene editing technology has significantly improved the study of filamentous fungi, which in the past was laborious and time-consuming. But recently, CRISPR-Cas systems, which utilize small guide RNA (sgRNA) to mediate clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas), have demonstrated considerable promise in research and application for filamentous fungi. The principle, function, and classification of CRISPR-Cas, along with its application strategies and research progress in filamentous fungi, will all be covered in the review. Additionally, we will go over general matters to take into account when editing a genome with the CRISPR-Cas system, including the creation of vectors, different transformation methodologies, multiple editing approaches, CRISPR-mediated transcriptional activation (CRISPRa) or interference (CRISPRi), base editors (BEs), and Prime editors (PEs).
dc.language.isoEN
dc.publisherFrontiers Media S.A.
dc.subject.lccMicrobiology
dc.titleUtilization of CRISPR-Cas genome editing technology in filamentous fungi: function and advancement potentiality
dc.typeArticle
dc.description.keywordsfilamentous fungi
dc.description.keywordsCRISPR-Cas
dc.description.keywordsgene editing
dc.description.keywordsCRISPR-dCas
dc.description.keywordsbase editors
dc.description.keywordsPrime editors
dc.description.doi10.3389/fmicb.2024.1375120
dc.title.journalFrontiers in Microbiology
dc.identifier.e-issn1664-302X
dc.identifier.oaioai:doaj.org/journal:19e4b44cde0845a99da483edf99cf10f


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