Background: Lung tuberculosis is a contagious disease that is still a public health problem. Indonesia is the country with the second most frequent tuberculosis cases in the world. One of the element that play a role in tuberculosis control is qualified human resources, including health cadres. Objective: To determine the effectiveness of training to increase knowledge and change the attitude of the health cadres against tuberculosis in Gunung Kidul. Method: This study uses the Quasi-Experiment research design, with a pretest-posttest design. There were 70 health cadres, 35 were given intervention (training) and 35 as controls. Before the given intervention is given a pretest. Intervention in the form of training for the cadres to help find out the suspect of Lung Tuberculosis patients in the community settings using a standard module of tuberculosis training. Posttest is administered at the end of the training. Analysis and test significance were performed using t-test paired and independent sample. Result: There was not significant difference between the trained and untrained cadres during the pretest (t-test independent sample, p> 0.05). There was a significant increase in knowledge in trained cadres (t-test paired sample, p < 0.05,) and there was a difference in the level of knowledge between trained and untrained cadres (t-test independent sample, p < 0.05), there was a change in attitude on the trained and untrained cadres (p < 0.05). Conclusion: With the training, it can be improve knowledge and influence the attitude of health cadres against tuberculosis.