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Assessment of <sup>18</sup>F-PBR-111 in the Cuprizone Mouse Model of Multiple Sclerosis

dc.contributor.authorValerie L. Jewells
dc.contributor.authorHong Yuan
dc.contributor.authorJoseph R. Merrill
dc.contributor.authorJonathan E. Frank
dc.contributor.authorAkhil Patel
dc.contributor.authorStephanie M. Cohen
dc.contributor.authorBen Giglio
dc.contributor.authorNana Nikolaishvili Feinberg
dc.contributor.authorGlenn K. Matsushima
dc.contributor.authorZibo Li
dc.contributor.otherDepartment of Radiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherDepartment of Radiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherBiomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherBiomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherUNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherLineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherBiomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherLineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherUNC Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.contributor.otherDepartment of Radiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
dc.date.accessioned2021-04-28T00:04:26Z
dc.date.available2025-10-02T03:13:29Z
dc.date.issued01-04-2021
dc.identifier.issn-
dc.identifier.urihttps://www.mdpi.com/2075-4418/11/5/786
dc.description.abstractThe study aims to assess site assessment of the performance of <sup>18</sup>F-PBR-111 as a neuroinflammation marker in the cuprizone mouse model of multiple sclerosis (MS). <sup>18</sup>F-PBR-111 PET imaging has not been well evaluated in multiple sclerosis applications both in preclinical and clinical research. This study will help establish the potential utility of <sup>18</sup>F-PBR-111 PET in preclinical MS research and future animal and future human applications. <sup>18</sup>F-PBR-111 PET/CT was conducted at 3.5 weeks (<i>n</i> = 7) and 5.0 weeks (<i>n</i> = 7) after cuprizone treatment or sham control (<i>n</i> = 3) in the mouse model. A subgroup of mice underwent autoradiography with cryosectioned brain tissue. T2 weighted MRI was performed to obtain the brain structural data of each mouse. <sup>18</sup>F-PBR-111 uptake was assessed in multiple brain regions with PET and autoradiography images. The correlation between autoradiography and immunofluorescence staining of neuroinflammation (F4/80 and CD11b) was measured. Compared to control mice, significant <sup>18</sup>F-PBR-111 uptake in the corpus callosum (<i>p</i> < 0.001), striatum (caudate and internal capsule, <i>p</i> < 0.001), and hippocampus (<i>p</i> < 0.05) was identified with PET images at both 3.5 weeks and 5.0 weeks, and validated with autoradiography. No significant uptake differences were detected between 3.5 weeks and 5.0 weeks assessing these regions as a whole, although there was a trend of increased uptake at 5.0 weeks compared to 3.5 weeks in the CC. High <sup>18</sup>F-PBR-111 uptake regions correlated with microglial/macrophage locations by immunofluorescence staining with F4/80 and CD11b antibodies. <sup>18</sup>F-PBR-111 uptake in anatomic locations correlated with activated microglia at histology in the cuprizone mouse model of MS suggests that <sup>18</sup>F-PBR-111 has potential for in vivo evaluation of therapy response and potential for use in MS patients and animal studies.
dc.format-
dc.language.isoEN
dc.publisherMDPI AG
dc.relation.uri['https://www.ecice06.com/EN/column/column35.shtml', 'https://www.ecice06.com/EN/home', 'https://www.ecice06.com/EN/column/column40.shtml']
dc.rightsCC BY-NC-ND
dc.subject['software', 'artificial intelligence', 'computer hardware', 'pattern recognition', 'computer networks', 'computer processing', 'Computer engineering. Computer hardware', 'TK7885-7895', 'Computer software', 'QA76.75-76.765']
dc.subject.lccMedicine (General)
dc.titleAssessment of <sup>18</sup>F-PBR-111 in the Cuprizone Mouse Model of Multiple Sclerosis
dc.typeArticle
dc.description.keywords<sup>18</sup>F-PBR-111
dc.description.keywordscuprizone
dc.description.keywordsmultiple sclerosis
dc.description.keywordsmicroglia
dc.description.keywordsTSPO
dc.description.pages-
dc.description.doi10.3390/diagnostics11050786
dc.title.journalDiagnostics
dc.identifier.e-issn2075-4418
dc.identifier.oaioai:doaj.org/journal:7992682748b24d1a9691703d5d3c7c9f
dc.journal.infoVolume 11, Issue 5


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