Show simple item record

An Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples

dc.contributor.authorDaniele Obino
dc.contributor.authorMassimo Vassalli
dc.contributor.authorAlberto Franceschi
dc.contributor.authorAndrea Alessandrini
dc.contributor.authorPaolo Facci
dc.contributor.authorFederica Viti
dc.contributor.otherInstitute of Biophysics, National Research Council, 16149 Genova, Italy
dc.contributor.otherCentre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, James Watt South Building, Glasgow G128LT, UK
dc.contributor.otherDiagnostica Veterinaria La Lanterna S.n.c., 16149 Genova, Italy
dc.contributor.otherNanoscience Institute, National Research Council, 41125 Modena, Italy
dc.contributor.otherInstitute of Biophysics, National Research Council, 16149 Genova, Italy
dc.contributor.otherInstitute of Biophysics, National Research Council, 16149 Genova, Italy
dc.date.accessioned2021-04-28T00:06:53Z
dc.date.available2025-10-02T03:46:52Z
dc.date.issued01-04-2021
dc.identifier.issn-
dc.identifier.urihttps://www.mdpi.com/1424-8220/21/9/3058
dc.description.abstractNucleic acid (NA) extraction is a basic step for genetic analysis, from scientific research to diagnostic and forensic applications. It aims at preparing samples for its application with biomolecular technologies such as isothermal and non-isothermal amplification, hybridization, electrophoresis, Sanger sequencing and next-generation sequencing. Multiple steps are involved in NA collection from raw samples, including cell separation from the rest of the specimen, cell lysis, NA isolation and release. Typically, this process needs molecular biology facilities, specialized instrumentation and labor-intensive operations. Microfluidic devices have been developed to analyze NA samples with high efficacy and sensitivity. In this context, the integration within the chip of the sample preparation phase is crucial to leverage the promise of portable, fast, user-friendly and economic point-of-care solutions. This review presents an overview of existing lab-on-a-chip (LOC) solutions designed to provide automated NA extraction from human raw biological fluids, such as whole blood, excreta (urine and feces), saliva. It mainly focuses on LOC implementation aspects, aiming to describe a detailed panorama of strategies implemented for different human raw sample preparations.
dc.format-
dc.language.isoEN
dc.publisherMDPI AG
dc.relation.uri['http://revistas.uned.es/index.php/ETFIII/about/submissions#authorGuidelines', 'http://revistas.uned.es/index.php/ETFIII/about/editorialPolicies#focusAndScope', 'http://revistas.uned.es/index.php/ETFIII']
dc.rightsCC BY-NC
dc.subject['medieval history', 'medieval studies', 'History (General) and history of Europe', 'D', 'History (General)', 'D1-2009', 'Medieval history', 'D111-203']
dc.subject.lccChemical technology
dc.titleAn Overview on Microfluidic Systems for Nucleic Acids Extraction from Human Raw Samples
dc.typeArticle
dc.description.keywordslab-on-chip
dc.description.keywordsLOC
dc.description.keywordsnucleic acid extraction
dc.description.keywordsmicrofluidics
dc.description.keywordssolid-phase extraction
dc.description.keywordsSPE
dc.description.pages-
dc.description.doi10.3390/s21093058
dc.title.journalSensors
dc.identifier.e-issn1424-8220
dc.identifier.oaioai:doaj.org/journal:9a128fac6980440680dd5a8d7a8f0df3
dc.journal.infoVolume 21, Issue 9


This item appears in the following Collection(s)

Show simple item record