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Liquid chromatography tandem mass spectrometry method for the estimation of lamotrigine in human plasma: Application to a pharmacokinetic study

dc.contributor.authorSantosh Ghatol
dc.contributor.authorVatsal Vithlani
dc.contributor.authorSanjay Gurule
dc.contributor.authorArshad Khuroo
dc.contributor.authorTausif Monif
dc.contributor.authorPankaj Partani
dc.contributor.otherDepartment of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.contributor.otherDepartment of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.contributor.otherDepartment of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.contributor.otherDepartment of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.contributor.otherDepartment of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.contributor.otherCorresponding author. Tel.: +91 124 4768130; fax: +91 124 4231002.; Department of Clinical Pharmacology and Pharmacokinetics, Ranbaxy Research Laboratories, Plot: GP-V, Sec-18, HSIIDC, Gurgaon 122015, Haryana, India
dc.date.accessioned2017-12-15T13:15:28Z
dc.date.available2025-10-02T03:51:16Z
dc.date.issued01-04-2013
dc.identifier.issn-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S2095177912001086
dc.description.abstractA reliable, selective and sensitive liquid chromatography tandem mass spectrometry method was developed and validated for the quantification of lamotrigine in human plasma using lamotrigine-13C3, d3 as an internal standard. Analyte and internal standard were extracted from human plasma by solid-phase extraction and detected in positive ion mode by tandem mass spectrometry with electrospray ionization (ESI) interface. Chromatographic separation was performed on a Chromolith® SpeedROD; RP-18e column (50â 4.6 mm i.d.) using acetonitrile: 5±0.1 mM ammonium formate solution (90:10, v/v) as the mobile phase at a flow rate of 0.500 mL/min. The calibration curves were linear over the range of 5.02â 1226.47 ng/mL with the lower limit of quantitation validated at 5.02 ng/mL. The analytes were found stable in human plasma through three freeze (â 20 °C)-thaw (ice-cold water bath) cycles and under storage on bench-top in ice-cold water bath for at least 6.8 h, and also in the mobile phase at 10 °C for at least 57 h. The method has shown good reproducibility, as the intra- and inter-day precisions were within 3.0%, while the accuracies were within ±6.0% of nominal values. The validated LCâ MS/MS method was applied for the evaluation of pharmacokinetic and bioequivalence parameters of lamotrigine after an oral administration of 50 mg lamotrigine tablet to thirty-two healthy adult male volunteers. Keywords: Lamotrigine, Liquid chromatography/tandem mass spectrometry, Solid phase extraction, Pharmacokinetic study
dc.format-
dc.language.isoEN
dc.publisherElsevier
dc.relation.uri['https://jurnalfuf.uinsa.ac.id/index.php/religio', 'https://jurnalfuf.uinsa.ac.id/index.php/religio/guidelines', 'https://jurnalfuf.uinsa.ac.id/index.php/religio/scope']
dc.rightsCC BY
dc.subject['religion', 'Religion (General)', 'BL1-50', 'History and principles of religions', 'BL660-2680']
dc.subject.lccTherapeutics. Pharmacology
dc.titleLiquid chromatography tandem mass spectrometry method for the estimation of lamotrigine in human plasma: Application to a pharmacokinetic study
dc.typeArticle
dc.description.pages75-83
dc.description.doi10.1016/j.jpha.2012.09.001
dc.title.journalJournal of Pharmaceutical Analysis
dc.identifier.e-issn-
dc.identifier.oaioai:doaj.org/journal:18d819fa5a384bad9ff1b42dc8102db5
dc.journal.infoVolume 3, Issue 2


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