| dc.contributor.author | Bogdan Bucur | |
| dc.contributor.author | Cristina Purcarea | |
| dc.contributor.author | Silvana Andreescu | |
| dc.contributor.author | Alina Vasilescu | |
| dc.contributor.other | National Institute for Research and Development in Biological Sciences, 296 Splaiul Independentei, 060031 Bucharest, Romania | |
| dc.contributor.other | Institute of Biology, 296 Splaiul Independentei, 060031 Bucharest, Romania | |
| dc.contributor.other | Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13676, USA | |
| dc.contributor.other | International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania | |
| dc.date.accessioned | 2021-04-27T00:03:57Z | |
| dc.date.available | 2025-10-02T03:53:06Z | |
| dc.date.issued | 01-04-2021 | |
| dc.identifier.issn | - | |
| dc.identifier.uri | https://www.mdpi.com/1424-8220/21/9/3038 | |
| dc.description.abstract | Enzymatic biosensors enjoy commercial success and are the subject of continued research efforts to widen their range of practical application. For these biosensors to reach their full potential, their selectivity challenges need to be addressed by comprehensive, solid approaches. This review discusses the status of enzymatic biosensors in achieving accurate and selective measurements via direct biocatalytic and inhibition-based detection, with a focus on electrochemical enzyme biosensors. Examples of practical solutions for tackling the activity and selectivity problems and preventing interferences from co-existing electroactive compounds in the samples are provided such as the use of permselective membranes, sentinel sensors and coupled multi-enzyme systems. The effect of activators, inhibitors or enzymatic substrates are also addressed by coupled enzymatic reactions and multi-sensor arrays combined with data interpretation via chemometrics. In addition to these more traditional approaches, the review discusses some ingenious recent approaches, detailing also on possible solutions involving the use of nanomaterials to ensuring the biosensors’ selectivity. Overall, the examples presented illustrate the various tools available when developing enzyme biosensors for new applications and stress the necessity to more comprehensively investigate their selectivity and validate the biosensors versus standard analytical methods. | |
| dc.format | - | |
| dc.language.iso | EN | |
| dc.publisher | MDPI AG | |
| dc.relation.uri | ['https://matlab.labapress.com/matlab/author_guideline', 'https://matlab.labapress.com/matlab/', 'https://matlab.labapress.com/matlab/overview'] | |
| dc.rights | CC BY | |
| dc.subject | ['materials science', 'nanotechnology', 'liquid crystals', 'semiconductors', 'Mechanics of engineering. Applied mechanics', 'TA349-359', 'Technology (General)', 'T1-995'] | |
| dc.subject.lcc | Chemical technology | |
| dc.title | Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives | |
| dc.type | Article | |
| dc.description.keywords | selectivity | |
| dc.description.keywords | enzyme | |
| dc.description.keywords | electrochemical biosensor | |
| dc.description.keywords | enzymatic inhibition | |
| dc.description.keywords | biocatalytic sensor | |
| dc.description.pages | - | |
| dc.description.doi | 10.3390/s21093038 | |
| dc.title.journal | Sensors | |
| dc.identifier.e-issn | 1424-8220 | |
| dc.identifier.oai | oai:doaj.org/journal:17e79e574b664f7eadc4f57fe5dcab92 | |
| dc.journal.info | Volume 21, Issue 9 | |
