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Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (<i>Holothuria scabra</i>), Obtained through Dialysis and the Ultrafiltration Membrane

dc.contributor.authorSuryani Saallah
dc.contributor.authorJumardi Roslan
dc.contributor.authorFlavian Sheryl Julius
dc.contributor.authorSharinee Saallah
dc.contributor.authorUmi Hartina Mohamad Razali
dc.contributor.authorWolyna Pindi
dc.contributor.authorMohd Rosni Sulaiman
dc.contributor.authorKhairul Faizal Pa’ee
dc.contributor.authorSiti Mazlina Mustapa Kamal
dc.contributor.otherBiotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
dc.contributor.otherSection of Food Engineering Technology, Malaysian Institute of Chemical and Bioengineering Technology, Universiti Kuala Lumpur, Alor Gajah 78000, Malacca, Malaysia
dc.contributor.otherDepartment of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, UPM Serdang, Shah Alam 43400, Selangor, Malaysia
dc.date.accessioned2021-04-29T00:01:13Z
dc.date.available2025-10-02T04:07:11Z
dc.date.issued01-04-2021
dc.identifier.issn-
dc.identifier.urihttps://www.mdpi.com/1420-3049/26/9/2564
dc.description.abstractCollagen was extracted from the body wall of sea cucumber (<i>Holothuria scabra</i>) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.
dc.format-
dc.language.isoEN
dc.publisherMDPI AG
dc.relation.uri['https://www.sciencedirect.com/journal/ain-shams-engineering-journal', 'https://www.elsevier.com/authors/open-access/choice#waivers', 'https://www.sciencedirect.com/journal/ain-shams-engineering-journal/publish/guide-for-authors']
dc.rights['CC BY', 'CC BY-NC-ND']
dc.subject['mechanical engineering and electrical engineering', 'civil engineering', 'chemical engineering', 'petroleum engineering', 'environmental engineering', 'architectural and urban planning engineering', 'Engineering (General). Civil engineering (General)', 'TA1-2040']
dc.subject.lccOrganic chemistry
dc.titleComparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (<i>Holothuria scabra</i>), Obtained through Dialysis and the Ultrafiltration Membrane
dc.typeArticle
dc.description.keywordssea cucumber
dc.description.keywordscollagen
dc.description.keywordsdialysis
dc.description.keywordsultrafiltration membrane
dc.description.keywordsphysicochemical properties
dc.description.pages-
dc.description.doi10.3390/molecules26092564
dc.title.journalMolecules
dc.identifier.e-issn1420-3049
dc.identifier.oaioai:doaj.org/journal:9037e03c50d54d3b840610277235155e
dc.journal.infoVolume 26, Issue 9


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