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3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System

dc.contributor.authorJennifer Rosendahl
dc.contributor.authorAndreas Svanström
dc.contributor.authorMattias Berglin
dc.contributor.authorSarunas Petronis
dc.contributor.authorYalda Bogestål
dc.contributor.authorPatrik Stenlund
dc.contributor.authorSimon Standoft
dc.contributor.authorAnders Ståhlberg
dc.contributor.authorGöran Landberg
dc.contributor.authorGary Chinga-Carrasco
dc.contributor.authorJoakim Håkansson
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, Sweden
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.contributor.otherSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, Sweden
dc.contributor.otherSahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Box 425, Medicinaregatan 1G, SE-41390 Gothenburg, Sweden
dc.contributor.otherRISE PFI AS, Høgskoleringen 6b, NO-7491 Trondheim, Norway
dc.contributor.otherUnit of Biological Function, Division Materials and Production, RISE Research Institutes of Sweden, Box 857, SE-50115 Borås, Sweden
dc.date.accessioned2021-07-23T14:30:57Z
dc.date.available2025-10-02T04:43:28Z
dc.date.issued01-07-2021
dc.identifier.issn-
dc.identifier.urihttps://www.mdpi.com/2306-5354/8/7/97
dc.description.abstractCurrent conventional cancer drug screening models based on two-dimensional (2D) cell culture have several flaws and there is a large need of more in vivo mimicking preclinical drug screening platforms. The microenvironment is crucial for the cells to adapt relevant in vivo characteristics and here we introduce a new cell culture system based on three-dimensional (3D) printed scaffolds using cellulose nanofibrils (CNF) pre-treated with 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO) as the structural material component. Breast cancer cell lines, MCF7 and MDA-MB-231, were cultured in 3D TEMPO-CNF scaffolds and were shown by scanning electron microscopy (SEM) and histochemistry to grow in multiple layers as a heterogenous cell population with different morphologies, contrasting 2D cultured mono-layered cells with a morphologically homogenous cell population. Gene expression analysis demonstrated that 3D TEMPO-CNF scaffolds induced elevation of the stemness marker <i>CD44</i> and the migration markers <i>VIM</i> and <i>SNAI1</i> in MCF7 cells relative to 2D control. T47D cells confirmed the increased level of the stemness marker <i>CD44</i> and migration marker <i>VIM</i> which was further supported by increased capacity of holoclone formation for 3D cultured cells. Therefore, TEMPO-CNF was shown to represent a promising material for 3D cell culture model systems for cancer cell applications such as drug screening.
dc.format-
dc.language.isoEN
dc.publisherMDPI AG
dc.relation.uri['https://www.springernature.com/gp/open-research/policies/journal-policies/apc-waiver-countries', 'https://sustainableearthreviews.biomedcentral.com/submission-guidelines', 'https://sustainableearthreviews.biomedcentral.com/about', 'https://sustainableearthreviews.biomedcentral.com/']
dc.rights['CC BY', 'CC BY-NC-ND']
dc.subject['sustainability', 'sustainable development goals', 'Economic growth, development, planning', 'HD72-88']
dc.subject.lccTechnology
dc.title3D Printed Nanocellulose Scaffolds as a Cancer Cell Culture Model System
dc.typeArticle
dc.description.keywordsnanocellulose
dc.description.keywords3D printing
dc.description.keywordscancer
dc.description.keywords3D cell culture
dc.description.keywordsCNF
dc.description.keywordscancer stemness
dc.description.pages-
dc.description.doi10.3390/bioengineering8070097
dc.title.journalBioengineering
dc.identifier.e-issn2306-5354
dc.identifier.oaioai:doaj.org/journal:ec6b843993de405d9503480422ef04b9
dc.journal.infoVolume 8, Issue 7


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