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Current status of oxacillin-susceptible mecA-positive Staphylococcus aureus infection in Shanghai, China: A multicenter study

dc.contributor.authorJun-Lan Liu
dc.contributor.authorTian-Ming Li
dc.contributor.authorNi Zhong
dc.contributor.authorXing Wang
dc.contributor.authorJie Jiang
dc.contributor.authorWen-Xia Zhang
dc.contributor.authorRong Tang
dc.contributor.authorYin-Juan Guo
dc.contributor.authorYun Liu
dc.contributor.authorJiang Hu
dc.contributor.authorLi-Hua He
dc.contributor.authorJin Tang
dc.contributor.authorWen-Juan Wu
dc.contributor.authorMin Li
dc.contributor.otherDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
dc.contributor.otherDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China
dc.contributor.otherDepartment of Clinical Laboratory, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai Traditional Chinese Medicine University Affiliated Shuguang Hospital, Shanghai 201203, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China
dc.contributor.otherDepartment of Clinical Laboratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China
dc.contributor.otherDepartment of Laboratory Diagnostics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai Putuo District Liqun Hospital, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China; Corresponding author. Yishan Road No. 600, Shanghai 200233, China.
dc.contributor.otherDepartment of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China; Corresponding author. Yuntai Road No. 1800, Shanghai 200123, China.
dc.contributor.otherDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China; Corresponding author. Pujian Road No. 160, Shanghai 200127, China. Fax: +86 021 68383297.
dc.date.accessioned2021-12-16T06:07:11Z
dc.date.available2025-10-02T05:19:52Z
dc.date.issued01-12-2021
dc.identifier.issn-
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S168411822030178X
dc.description.abstractBackground: Oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) represents an important issue, as its oxacillin susceptibility has contributed to misidentification by conventional susceptibility tests and consequently potential therapeutic failure, but limited data on the current status of OS-MRSA infection in Chinese hospitals are available. Methods: This multicenter study performed a battery of susceptibility tests and diagnostic tests for 956 S. aureus isolates from 10 hospitals, including automated susceptibility testing on VITEK 2, broth microdilution, disk diffusion, and detection of PBB2a, mecA gene and mecC gene. For all identified OS-MRSA, multi-locus sequence typing (MLST), together with spa typing, SCCmec typing and PVL detecting, was carried out. Results: OS-MRSA, most of which were from pediatric inpatients, represented 1.8% (17/956) of total isolates. Of these 17 OS-MRSA, 10 were ST59, followed by ST965 (3/17), and 11 carried SCCmec type IV, while 5 carried SCCmec type V, but only one was Panton–Valentine leucocidin (PVL)-positive, also, 16 had one or two point mutations within mecA promoter. OS-MRSA had inducible oxacillin resistance and significantly lower MDR (Multi-Drug Resistant) rate. We observed that the VITEK 2 system exhibited some deficiency in OS-MRSA detection, whereas cefoxitin disk diffusion was shown to be a reliable and cost-saving alternative and should be supplemented in detecting S. aureus with borderline oxacillin susceptible MICs. Conclusion: This study has characterized phenotypically and molecularly OS-MRSA in China, and provided insights into more effective management of OS-MRSA.
dc.format-
dc.language.isoEN
dc.publisherElsevier
dc.relation.uri['https://www.springernature.com/gp/open-research/policies/journal-policies/apc-waiver-countries', 'https://cmbl.biomedcentral.com/submission-guidelines', 'https://cmbl.biomedcentral.com/', 'https://cmbl.biomedcentral.com/about']
dc.rightsCC BY
dc.subject['cancer cell biology', 'cell differentiation', 'cell cycle', 'stem cells', 'cellular membranes', 'molecular biotechnology', 'Cytology', 'QH573-671']
dc.subject.lccMicrobiology
dc.titleCurrent status of oxacillin-susceptible mecA-positive Staphylococcus aureus infection in Shanghai, China: A multicenter study
dc.typeArticle
dc.description.keywordsOS-MRSA
dc.description.keywordsAntimicrobial susceptibility tests
dc.description.keywordsVITEK 2
dc.description.keywordsStaphylococcus aureus
dc.description.pages1070-1077
dc.description.doi10.1016/j.jmii.2020.07.021
dc.title.journalJournal of Microbiology, Immunology and Infection
dc.identifier.e-issn-
dc.identifier.oaioai:doaj.org/journal:242379ac58f34acb8564d6afd096f147
dc.journal.infoVolume 54, Issue 6


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