| dc.contributor.author | Sang Koo Park | |
| dc.contributor.author | Yoon Kyung Lee | |
| dc.contributor.other | Food Safety Management Division, Seoul Regional Korea Food and Drug Administration, Seoul 07978, Korea | |
| dc.contributor.other | Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea | |
| dc.date.accessioned | 2021-04-28T00:07:09Z | |
| dc.date.available | 2025-10-02T05:20:37Z | |
| dc.date.issued | 01-04-2021 | |
| dc.identifier.issn | - | |
| dc.identifier.uri | https://www.mdpi.com/1420-3049/26/9/2555 | |
| dc.description.abstract | Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, <i>Rheum emodi</i> wall, was measured and the antioxidant activity was determined using DPPH and ABTS<sup>+</sup> assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, <i>n</i>-hexane, <i>n</i>-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for <i>R. emodi</i> fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS<sup>+</sup> assay results are presented into IC<sub>50</sub> values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, <i>n</i>-butanol and water fractions showed significantly lower IC<sub>50</sub> values than the positive control in DPPH radical scavenging activity. The IC<sub>50</sub> values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of <i>R. emodi</i> possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of <i>R. emodi</i> were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about <i>R. emodi</i> before. | |
| dc.format | - | |
| dc.language.iso | EN | |
| dc.publisher | MDPI AG | |
| dc.relation.uri | ['https://www.springernature.com/gp/open-research/policies/journal-policies/apc-waiver-countries', 'https://cellandbioscience.biomedcentral.com/submission-guidelines', 'https://cellandbioscience.biomedcentral.com/about', 'https://cellandbioscience.biomedcentral.com/'] | |
| dc.rights | ['CC BY', 'CC0'] | |
| dc.subject | ['life sciences', 'biology', 'biotechnology', 'biochemistry', 'pharmacology', 'genetics', 'Biotechnology', 'TP248.13-248.65', 'Biology (General)', 'QH301-705.5', 'Biochemistry', 'QD415-436'] | |
| dc.subject.lcc | Organic chemistry | |
| dc.title | Antioxidant Activity in <i>Rheum emodi</i> Wall (Himalayan Rhubarb) | |
| dc.type | Article | |
| dc.description.keywords | <i>Rheum emodi</i> wall | |
| dc.description.keywords | himalayan rhubarb | |
| dc.description.keywords | polyphenol content | |
| dc.description.keywords | antioxidant activity | |
| dc.description.keywords | DPPH | |
| dc.description.keywords | SOD | |
| dc.description.pages | - | |
| dc.description.doi | 10.3390/molecules26092555 | |
| dc.title.journal | Molecules | |
| dc.identifier.e-issn | 1420-3049 | |
| dc.identifier.oai | oai:doaj.org/journal:716deddeb26d4a2f8a35ee9bfce7fd8d | |
| dc.journal.info | Volume 26, Issue 9 | |
