Show simple item record

Colorectal cancer microbiome programs DNA methylation of host cells by affecting methyl donor metabolism

dc.contributor.authorZhi Liu
dc.contributor.authorQingqing Zhang
dc.contributor.authorHong Zhang
dc.contributor.authorZhongyuan Yi
dc.contributor.authorHuihui Ma
dc.contributor.authorXiaoyi Wang
dc.contributor.authorJingjing Wang
dc.contributor.authorYang Liu
dc.contributor.authorYi Zheng
dc.contributor.authorWeijia Fang
dc.contributor.authorPing Huang
dc.contributor.authorXingyin Liu
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherCore Facility Center, The First Affiliated Hospital of Nanjing Medical University
dc.contributor.otherThe Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.contributor.otherDepartment of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine
dc.contributor.otherDepartment of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine
dc.contributor.otherDepartment of Surgery, The Third Affiliated Hospital, Nanjing Medical University
dc.contributor.otherDepartment of Pathogen Biology-Microbiology Division, State Key Laboratory of Reproductive Medicine, Key Laboratory of Pathogen of Jiangsu Province, Key Laboratory of Human Functional Genomics of Jiangsu Province, Center of Global Health, Nanjing Medical University
dc.date.accessioned2024-06-09T11:28:25Z
dc.date.accessioned2025-10-08T08:22:45Z
dc.date.available2025-10-08T08:22:45Z
dc.date.issued01-06-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/35664
dc.description.abstractAbstract Background Colorectal cancer (CRC) arises from complex interactions between host and environment, which include the gut and tissue microbiome. It is hypothesized that epigenetic regulation by gut microbiota is a fundamental interface by which commensal microbes dynamically influence intestinal biology. The aim of this study is to explore the interplay between gut and tissue microbiota and host DNA methylation in CRC. Methods Metagenomic sequencing of fecal samples was performed on matched CRC patients (n = 18) and healthy controls (n = 18). Additionally, tissue microbiome was profiled with 16S rRNA gene sequencing on tumor (n = 24) and tumor-adjacent normal (n = 24) tissues of CRC patients, while host DNA methylation was assessed through whole-genome bisulfite sequencing (WGBS) in a subset of 13 individuals. Results Our analysis revealed substantial alterations in the DNA methylome of CRC tissues compared to adjacent normal tissues. An extensive meta-analysis, incorporating publicly available and in-house data, identified significant shifts in microbial-derived methyl donor-related pathways between tumor and adjacent normal tissues. Of note, we observed a pronounced enrichment of microbial-associated CpGs within the promoter regions of genes in adjacent normal tissues, a phenomenon notably absent in tumor tissues. Furthermore, we established consistent and recurring associations between methylation patterns of tumor-related genes and specific bacterial taxa. Conclusions This study emphasizes the pivotal role of the gut microbiota and pathogenic bacteria in dynamically shaping DNA methylation patterns, impacting physiological homeostasis, and contributing to CRC tumorigenesis. These findings provide valuable insights into the intricate host-environment interactions in CRC development and offer potential avenues for therapeutic interventions in this disease.
dc.language.isoEN
dc.publisherBMC
dc.subject.lccMedicine
dc.titleColorectal cancer microbiome programs DNA methylation of host cells by affecting methyl donor metabolism
dc.typeArticle
dc.description.keywordsColorectal cancer
dc.description.keywordsDNA methylation
dc.description.keywordsMicrobiota
dc.description.keywordsMethionine metabolism
dc.description.pages1-19
dc.description.doi10.1186/s13073-024-01344-1
dc.title.journalGenome Medicine
dc.identifier.e-issn1756-994X
dc.identifier.oai4c130381dd3d457291d798d79b00c00d
dc.journal.infoVolume 16, Issue 1


This item appears in the following Collection(s)

Show simple item record