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Evaluation of Bispecific T-Cell Engagers Targeting Murine Cytomegalovirus

dc.contributor.authorHanna Menschikowski
dc.contributor.authorChristopher Bednar
dc.contributor.authorSabrina Kübel
dc.contributor.authorManuel Hermann
dc.contributor.authorLarissa Bauer
dc.contributor.authorMarco Thomas
dc.contributor.authorArne Cordsmeier
dc.contributor.authorArmin Ensser
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.contributor.otherInstitute of Clinical and Molecular Virology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
dc.date.accessioned2024-06-26T15:36:10Z
dc.date.accessioned2025-10-08T08:24:12Z
dc.date.available2025-10-08T08:24:12Z
dc.date.issued01-05-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/35767
dc.description.abstractHuman cytomegalovirus is a ubiquitous herpesvirus that, while latent in most individuals, poses a great risk to immunocompromised patients. In contrast to directly acting traditional antiviral drugs, such as ganciclovir, we aim to emulate a physiological infection control using T cells. For this, we constructed several bispecific T-cell engager (BiTE) constructs targeting different viral glycoproteins of the murine cytomegalovirus and evaluated them in vitro for their efficacy. To isolate the target specific effect without viral immune evasion, we established stable reporter cell lines expressing the viral target glycoprotein B, and the glycoprotein complexes gN-gM and gH-gL, as well as nano-luciferase (nLuc). First, we evaluated binding capacities using flow cytometry and established killing assays, measuring nLuc-release upon cell lysis. All BiTE constructs proved to be functional mediators for T-cell recruitment and will allow a proof of concept for this treatment option. This might pave the way for strikingly safer immunosuppression in vulnerable patient groups.
dc.language.isoEN
dc.publisherMDPI AG
dc.subject.lccMicrobiology
dc.titleEvaluation of Bispecific T-Cell Engagers Targeting Murine Cytomegalovirus
dc.typeArticle
dc.description.keywordscytomegalovirus
dc.description.keywordsmurine cytomegalovirus
dc.description.keywordsCMV
dc.description.keywordsBiTE
dc.description.keywordsbispecific T-cell engager
dc.description.keywordsT cell
dc.description.doi10.3390/v16060869
dc.title.journalViruses
dc.identifier.e-issn1999-4915
dc.identifier.oai74e98477d1814c2fb4ee0a527c4c9581
dc.journal.infoVolume 16, Issue 6


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