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Applying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences Across HIV Model Systems

dc.contributor.authorJonathan Burnie
dc.contributor.authorClaire Fernandes
dc.contributor.authorAyushi Patel
dc.contributor.authorArvin Tejnarine Persaud
dc.contributor.authorDeepa Chaphekar
dc.contributor.authorDanlan Wei
dc.contributor.authorTimothy Kit Hin Lee
dc.contributor.authorVera A. Tang
dc.contributor.authorClaudia Cicala
dc.contributor.authorJames Arthos
dc.contributor.authorChristina Guzzo
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherLaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.contributor.otherFlow Cytometry and Virometry Core Facility, Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada
dc.contributor.otherLaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
dc.contributor.otherLaboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
dc.contributor.otherDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, ON M1C 1A4, Canada
dc.date.accessioned2024-06-26T15:38:52Z
dc.date.accessioned2025-10-08T08:24:17Z
dc.date.available2025-10-08T08:24:17Z
dc.date.issued01-06-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/35775
dc.description.abstractThe HIV envelope glycoprotein (Env) is a trimeric protein that facilitates viral binding and fusion with target cells. As the sole viral protein on the HIV surface, Env is important both for immune responses to HIV and in vaccine designs. Targeting Env in clinical applications is challenging due to its heavy glycosylation, high genetic variability, conformational camouflage, and its low abundance on virions. Thus, there is a critical need to better understand this protein. Flow virometry (FV) is a useful methodology for phenotyping the virion surface in a high-throughput, single virion manner. To demonstrate the utility of FV to characterize Env, we stained HIV virions with a panel of 85 monoclonal antibodies targeting different regions of Env. A broad range of antibodies yielded robust staining of Env, with V3 antibodies showing the highest quantitative staining. A subset of antibodies tested in parallel on viruses produced in CD4<sup>+</sup> T cell lines, HEK293T cells, and primary cells showed that the cellular model of virus production can impact Env detection. Finally, in addition to being able to highlight Env heterogeneity on virions, we show FV can sensitively detect differences in Env conformation when soluble CD4 is added to virions before staining.
dc.language.isoEN
dc.publisherMDPI AG
dc.subject.lccMicrobiology
dc.titleApplying Flow Virometry to Study the HIV Envelope Glycoprotein and Differences Across HIV Model Systems
dc.typeArticle
dc.description.keywordshuman immunodeficiency virus (HIV)
dc.description.keywordscalibrated flow virometry
dc.description.keywordsmolecules of equivalent soluble fluorophore (MESF)
dc.description.keywordsnanoscale flow cytometry
dc.description.keywordsgp120/gp41
dc.description.keywordsHIV Env
dc.description.doi10.3390/v16060935
dc.title.journalViruses
dc.identifier.e-issn1999-4915
dc.identifier.oaif0cbfc8da56341c1b3efe4c874ff5659
dc.journal.infoVolume 16, Issue 6


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