Show simple item record

Completing the view – histologic insights from circular AAA specimen including 3D imaging

dc.contributor.authorAnna-Leonie Menges
dc.contributor.authorMaja Nackenhorst
dc.contributor.authorJohannes R. Müller
dc.contributor.authorMarie-Luise Engl
dc.contributor.authorRenate Hegenloh
dc.contributor.authorJaroslav Pelisek
dc.contributor.authorEllen Geibelt
dc.contributor.authorAnja Hofmann
dc.contributor.authorChristian Reeps
dc.contributor.authorGabor Biro
dc.contributor.authorHans-Henning Eckstein
dc.contributor.authorAlexander Zimmermann
dc.contributor.authorDerek Magee
dc.contributor.authorMartin Falk
dc.contributor.authorNadja Sachs
dc.contributor.authorAlbert Busch
dc.contributor.otherDepartment for Vascular Surgery, University Hospital Zurich
dc.contributor.otherDepartment of Pathology, Medical University of Vienna
dc.contributor.otherDFG Cluster of Excellence “Physics of Life”, TU Dresden
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.contributor.otherDepartment for Vascular Surgery, University Hospital Zurich
dc.contributor.otherLight Microscopy Facility, Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität Dresden
dc.contributor.otherDepartment for Visceral-, Thoracic and Vascular Surgery, Medical Faculty and University Hospital Carl Gustav Carus, TUD Dresden University of Technology
dc.contributor.otherDepartment for Visceral-, Thoracic and Vascular Surgery, Medical Faculty and University Hospital Carl Gustav Carus, TUD Dresden University of Technology
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.contributor.otherDepartment for Vascular Surgery, University Hospital Zurich
dc.contributor.otherHeteroGenius Limited
dc.contributor.otherScientific Visualization Group, Department of Science and Technology (ITN), Linköping University
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.contributor.otherTechnical University Munich, Department for Vascular and Endovascular Surgery, Klinikum Rechts der Isar
dc.date.accessioned2023-06-18T11:05:03Z
dc.date.accessioned2025-10-08T08:27:23Z
dc.date.available2025-10-08T08:27:23Z
dc.date.issued01-06-2023
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/35952
dc.description.abstractAbstract Abdominal aortic aneurysm (AAA) is a pathologic enlargement of the infrarenal aorta with an associated risk of rupture. However, the responsible mechanisms are only partially understood. Based on murine and human samples, a heterogeneous distribution of characteristic pathologic features across the aneurysm circumference is expected. Yet, complete histologic workup of the aneurysm sac is scarcely reported. Here, samples from five AAAs covering the complete circumference partially as aortic rings are investigated by histologic means (HE, EvG, immunohistochemistry) and a new method embedding the complete ring. Additionally, two different methods of serial histologic section alignment are applied to create a 3D view. The typical histopathologic features of AAA, elastic fiber degradation, matrix remodeling with collagen deposition, calcification, inflammatory cell infiltration and thrombus coverage were distributed without recognizable pattern across the aneurysm sac in all five patients. Analysis of digitally scanned entire aortic rings facilitates the visualization of these observations. Immunohistochemistry is feasible in such specimen, however, tricky due to tissue disintegration. 3D image stacks were created using open-source and non-generic software correcting for non-rigid warping between consecutive sections. Secondly, 3D image viewers allowed visualization of in-depth changes of the investigated pathologic hallmarks. In conclusion, this exploratory descriptive study demonstrates a heterogeneous histomorphology around the AAA circumference. Warranting an increased sample size, these results might need to be considered in future mechanistic research, especially in reference to intraluminal thrombus coverage. 3D histology of such circular specimen could be a valuable visualization tool for further analysis.
dc.language.isoEN
dc.publisherBMC
dc.subject.lccPathology
dc.titleCompleting the view – histologic insights from circular AAA specimen including 3D imaging
dc.typeArticle
dc.description.keywordsAbdominal Aortic Aneurysm
dc.description.keywordsAneurysm sac
dc.description.keywords3D histology
dc.description.keywordsInflammation
dc.description.pages1-12
dc.description.doi10.1186/s13000-023-01359-z
dc.title.journalDiagnostic Pathology
dc.identifier.e-issn1746-1596
dc.identifier.oai67455cab17324b0c9f92219a7b03342b
dc.journal.infoVolume 18, Issue 1


This item appears in the following Collection(s)

Show simple item record