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Structure of an RNA G-quadruplex from the West Nile virus genome

dc.contributor.authorJ. Ross Terrell
dc.contributor.authorThao T. Le
dc.contributor.authorAnanya Paul
dc.contributor.authorMargo A. Brinton
dc.contributor.authorW. David Wilson
dc.contributor.authorGregory M. K. Poon
dc.contributor.authorMarkus W. Germann
dc.contributor.authorJessica L. Siemer
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Biology, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.contributor.otherDepartment of Chemistry, Georgia State University
dc.date.accessioned2024-06-30T11:27:03Z
dc.date.accessioned2025-10-08T08:39:30Z
dc.date.available2025-10-08T08:39:30Z
dc.date.issued01-06-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/36576
dc.description.abstractAbstract Potential G-quadruplex sites have been identified in the genomes of DNA and RNA viruses and proposed as regulatory elements. The genus Orthoflavivirus contains arthropod-transmitted, positive-sense, single-stranded RNA viruses that cause significant human disease globally. Computational studies have identified multiple potential G-quadruplex sites that are conserved across members of this genus. Subsequent biophysical studies established that some G-quadruplexes predicted in Zika and tickborne encephalitis virus genomes can form and known quadruplex binders reduced viral yields from cells infected with these viruses. The susceptibility of RNA to degradation and the variability of loop regions have made structure determination challenging. Despite these difficulties, we report a high-resolution structure of the NS5-B quadruplex from the West Nile virus genome. Analysis reveals two stacked tetrads that are further stabilized by a stacked triad and transient noncanonical base pairing. This structure expands the landscape of solved RNA quadruplex structures and demonstrates the diversity and complexity of biological quadruplexes. We anticipate that the availability of this structure will assist in solving further viral RNA quadruplexes and provides a model for a conserved antiviral target in Orthoflavivirus genomes.
dc.language.isoEN
dc.publisherNature Portfolio
dc.subject.lccScience
dc.titleStructure of an RNA G-quadruplex from the West Nile virus genome
dc.typeArticle
dc.description.pages1-10
dc.description.doi10.1038/s41467-024-49761-5
dc.title.journalNature Communications
dc.identifier.e-issn2041-1723
dc.identifier.oaioai:doaj.org/journal:8e9f01cae3524f5faf463f7f903561c0
dc.journal.infoVolume 15, Issue 1


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