It is critical to provide proper environmental conditions in poultry houses to maintain birds’ health, boost productivity, and improve the overall economic viability of the poultry industry. Among the myriad of environmental elements, indoor air quality has been a determining factor that directly affects poultry well-being. Elevated concentrations of harmful gases—in particular Carbon Dioxide (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula>), Methane (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><msub><mi>H</mi><mn>4</mn></msub></mrow></semantics></math></inline-formula>), and Ammonia (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>N</mi><msub><mi>H</mi><mn>3</mn></msub></mrow></semantics></math></inline-formula>)—decomposition products of poultry litter, feed wastage, and biological processes have draconian effects on bird health, feed efficiency, the growth rate, reproduction efficiency, and mortality rate. Despite their importance, traditional air quality monitoring systems are often operated manually, labor intensive, and cannot detect sudden environmental changes due to the lack of real-time sensing. To overcome these limitations, this paper presents an interdisciplinary approach combining cloud computing, Artificial Intelligence (AI), and Internet of Things (IoT) technologies to measure real-time poultry gas concentrations. Real-time sensor feeds are transmitted to a cloud-based platform, which stores, displays, and processes the data. Furthermore, a machine learning (ML) model was trained using historical sensory data to predict the next-day gas emission levels. A web-based platform has been developed to enable convenient user interaction and display the gas sensory readings on an interactive dashboard. Also, the developed system triggers automatic alerts when gas levels cross safe environmental thresholds. Experimental results of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> concentrations showed a significant diurnal trend, peaking in the afternoon, followed by the evening, and reaching their lowest levels in the morning. In particular, <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> concentrations peaked at approximately 570 ppm during the afternoon, a value that was significantly elevated (<i>p</i> < 0.001) compared to those recorded in the evening (~560 ppm) and morning (~555 ppm). This finding indicates a distinct diurnal pattern in <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> accumulation, with peak concentrations occurring during the warmer afternoon hours.