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Proper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation

dc.contributor.authorShuai Nie
dc.contributor.authorYong Yan
dc.contributor.authorYue Wang
dc.contributor.authorShanshan Liu
dc.contributor.authorWenhui Guo
dc.contributor.authorLing Yang
dc.contributor.authorLing Yang
dc.contributor.authorHailong Shen
dc.contributor.authorHailong Shen
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherState Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin, China
dc.contributor.otherCollege of Forestry, Northeast Forestry University, Harbin, China
dc.contributor.otherState Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine, Harbin, China
dc.date.accessioned2024-01-23T11:44:05Z
dc.date.accessioned2025-10-08T08:46:07Z
dc.date.available2025-10-08T08:46:07Z
dc.date.issued01-01-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/37067
dc.description.abstractSomatic embryogenesis of Korean pine (Pinus koraiensis Sieb. Et Zucc.), an ecologically and econimically very important conifer species, was hindered by the gradually weakens and fast runaway of the embryogenicity and embryo competence of the embryogenic callus. Brassinolide (BL) has shown the enhancing capability of somatic embryo regeneration. For checking the function of BL in this issue, we applied different concentrations of BL to Korean pine callus materials exhibiting different embryogenic capacities and subsequently monitored the physiological alterations and hormone dynamics of the embryogenic callus. Our study revealed that calli with different embryogenic strengths responded differently to different concentrations of BL, but the effect after the addition of BL was very uniform. The addition of BL during the proliferation phase of embryogenic callus may help to stimulate the biological activity of callus during the proliferation process and improve the level of cell metabolism, which is accompanied by a reduction in storage substances. BL could reduce the level of endogenous auxin IAA in embryogenic callus and increase the level of abscisic acid to regulate cell division and differentiation. In addition, the MDA content in the callus was significantly decreased and the activity of antioxidant enzymes was significantly increased after the addition of BL. During the proliferation of embryogenic callus, BL was added to participate in the metabolism of phenylpropane in the cells and to increase the activity of phenylalanine ammonia-lyase and the content of lignin in the cells. We deduced that the proper doses of BL for Korean pine embryogenic callus culture were as follow: calli with low, high and decreasing embryogenicity were subcultured after the addition of 0.75 mg/L, 0.35 mg/L, 2.00 mg/L BL, respectively, during proliferation culture stage.
dc.language.isoEN
dc.publisherFrontiers Media S.A.
dc.subject.lccPlant culture
dc.titleProper doses of brassinolide enhance somatic embryogenesis in different competent Korean pine cell lines during embryogenic callus differentiation
dc.typeArticle
dc.description.keywordsPinus koraiensis
dc.description.keywordsbrassinolides
dc.description.keywordsantioxidant enzymes
dc.description.keywordshormones
dc.description.keywordsembryogenic callus
dc.description.keywordssomatic embryogenesis
dc.description.doi10.3389/fpls.2024.1330103
dc.title.journalFrontiers in Plant Science
dc.identifier.e-issn1664-462X
dc.identifier.oaioai:doaj.org/journal:b613321bfbf147ffb162ab91077cd3d6


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