IntroductionSoluble solids content (SSC) is a pivotal parameter for assessing tomato quality. Traditional measurement methods are both destructive and time-consuming.MethodsTo enhance accuracy and efficiency in SSC assessment, this study employs full transmission visible and near-infrared (Vis-NIR) spectroscopy and multi-point spectral data collection techniques to quantitatively analyze SSC in two tomato varieties (‘Provence’ and ‘Jingcai No.8’ tomatoes). Preprocessing of the multi-point spectra is carried out using a weighted averaging approach, aimed at noise reduction, signal-to-noise ratio improvement, and overall data quality enhancement. Taking into account the potential influence of various detection orientations and preprocessing methods on model outcomes, we investigate the combination of partial least squares regression (PLSR) with two orientations (O1 and O2) and two preprocessing techniques (Savitzky-Golay smoothing (SG) and Standard Normal Variate transformation (SNV)) in the development of SSC prediction models.ResultsThe model achieved the best results in the O2 orientation and SNV pretreatment as follows: ‘Provence’ tomato (Rp = 0.81, RMSEP = 0.69°Brix) and ‘Jingcai No.8’ tomatoes (Rp = 0.84, RMSEP = 0.64°Brix). To further optimize the model, characteristic wavelength selection is introduced through Least Angle Regression (LARS) with L1 and L2 regularization. Notably, when λ=0.004, LARS-L1 produces superior results (‘Provence’ tomato: Rp = 0.95, RMSEP = 0.35°Brix; ‘Jingcai No.8’ tomato: Rp = 0.96, RMSEP = 0.33°Brix).DiscussionThis study underscores the effectiveness of full transmission Vis-NIR spectroscopy in predicting SSC in different tomato varieties, offering a viable method for accurate and swift SSC assessment in tomatoes.