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Patulin induces ROS-dependent cardiac cell toxicity by inducing DNA damage and activating endoplasmic reticulum stress apoptotic pathway

dc.contributor.authorBaigang Zhang
dc.contributor.authorChenghui Huang
dc.contributor.authorDongmei Xu
dc.contributor.authorKe Huang
dc.contributor.authorYang Li
dc.contributor.authorLu Jiao
dc.contributor.authorBinggang Fu
dc.contributor.authorSubing Li
dc.contributor.authorYi Li
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherKey Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730030, China; Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou 730030, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherLife Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
dc.contributor.otherKey Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Lanzhou 730030, China; Corresponding author.
dc.date.accessioned2023-12-07T05:27:29Z
dc.date.accessioned2025-10-08T08:48:51Z
dc.date.available2025-10-08T08:48:51Z
dc.date.issued01-01-2024
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/37430
dc.description.abstractPatulin (PAT) is one of the mycotoxins commonly found in agricultural products and fruits, and has obvious toxic effects on animals and humans. PAT has been found to cause myocardial toxicity and oxidative damage, but the mechanism of myocardial toxicity remained to be elucidated. We investigated the toxic effects and potential mechanisms of PAT on human cardiomyocytes and explored the effects of reactive oxygen species (ROS) on them. The study showed that treatment with PAT for 24 h decreased cell viability and superoxide dismutase (SOD) activity, and increased ROS and lactate dehydrogenase (LDH) levels. Moreover, in addition to detecting increased γ-H2AX expression and observing nuclear damage, the comet assay also showed increased DNA tail distance in the PAT-treated group, followed by an increase in phosphorylation of the p53 protein and p21 protein expression, and a decrease in CDK1 and Cyclin B1 protein expression, and G2/M phase arrest. In addition, PAT induced endoplasmic reticulum stress (ERS) and induced apoptosis, as evidenced by Ca2+ increase, ER enlargement and swelling, and upregulation of ERS-related genes and proteins expression, and increased expression of three apoptotic pathway proteins under ERS, including CHOP, JNK, and caspase-12. Meanwhile, N-acetylcysteine (NAC, a ROS scavenger) reversed the negative effects of PAT treatment on cells. These results clarify that excessive ROS production by PAT-treated AC16 cells not only causes DNA damage, leading to cell cycle arrest, but also causes ERS, which triggers apoptotic pathways to cause apoptosis.
dc.language.isoEN
dc.publisherElsevier
dc.subject.lccEnvironmental pollution
dc.titlePatulin induces ROS-dependent cardiac cell toxicity by inducing DNA damage and activating endoplasmic reticulum stress apoptotic pathway
dc.typeArticle
dc.description.keywordsPatulin
dc.description.keywordsCycle arrest
dc.description.keywordsDNA damage
dc.description.keywordsEndoplasmic reticulum stress
dc.description.keywordsApoptosis
dc.description.doi10.1016/j.ecoenv.2023.115784
dc.title.journalEcotoxicology and Environmental Safety
dc.identifier.oaioai:doaj.org/journal:df84ed1c65614949bd8157919bfb4490


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