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A Microfluidic System for Real-Time Monitoring and In Situ Metabolite Detection of Plasma-Enhanced Wound Healing

dc.contributor.authorZujie Gao
dc.contributor.authorJinlong Xu
dc.contributor.authorHengxin Zhao
dc.contributor.authorXiaobing Zheng
dc.contributor.authorZijian Lyu
dc.contributor.authorQiwei Liu
dc.contributor.authorHao Chen
dc.contributor.authorYu Zhang
dc.contributor.authorHe-Ping Li
dc.contributor.authorYongjian Li
dc.contributor.otherDepartment of Mechanical Engineering, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Mechanical Engineering, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Engineering Physics, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Mechanical Engineering, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Engineering Physics, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Mechanical Engineering, Tsinghua University, Beijing 100084, China
dc.contributor.otherSchool of Clinical Medicine, Tsinghua University, Beijing 100084, China
dc.contributor.otherSchool of Basic Medical Sciences, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Engineering Physics, Tsinghua University, Beijing 100084, China
dc.contributor.otherDepartment of Mechanical Engineering, Tsinghua University, Beijing 100084, China
dc.date.accessioned2025-08-27T14:15:18Z
dc.date.accessioned2025-10-08T08:55:50Z
dc.date.available2025-10-08T08:55:50Z
dc.date.issued01-07-2025
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/38178
dc.description.abstractAlthough cold atmospheric plasma (CAP) has shown promise in facilitating wound repair due to its non-thermal and non-invasive properties, its dynamic effects on cellular response and metabolic regulation remain poorly characterized, and the mechanism is still unclear. In this study, we developed a microfluidic experimental system that integrates a CAP treatment module with multiparametric in situ sensing capabilities, along with precise environmental control of temperature, humidity, and CO<sub>2</sub> concentration. A stratified microfluidic chip was engineered to co-culture HaCaT keratinocytes and HSF fibroblasts. CAP treatment was applied within this platform, and the dynamic processes of cell migration, proliferation, and multiple metabolic markers were simultaneously monitored. The experimental results show that the system can not only achieve real-time observation in the healing process under plasma intervention, but also find that the healing process is closely related to the concentration of NO<sub>2</sub><sup>−</sup>. In addition, the study also found that keratin KRT14, which is thought to be closely related to wound healing, decreased significantly in the process of plasma-induced healing. The platform provides high-resolution experimental tools to elucidate the biological effects of CAP and has the potential for parameter optimization, material evaluation, and personalized therapeutic development to advance plasma research and clinical translational applications.
dc.language.isoEN
dc.publisherMDPI AG
dc.subject.lccMicrobiology
dc.titleA Microfluidic System for Real-Time Monitoring and In Situ Metabolite Detection of Plasma-Enhanced Wound Healing
dc.typeArticle
dc.description.keywordswound healing
dc.description.keywordscold atmospheric plasma
dc.description.keywordsmicrofluidic chip
dc.description.keywordsmultiparametric sensing
dc.description.keywordsmetabolic dynamic monitoring
dc.description.doi10.3390/biom15081077
dc.title.journalBiomolecules
dc.identifier.e-issn2218-273X
dc.identifier.oaioai:doaj.org/journal:367bd83dc300470cae1c965e3c3ab3df
dc.journal.infoVolume 15, Issue 8


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