| dc.contributor.author | Chenhan Li | |
| dc.contributor.author | Shun-ichiro Ogura | |
| dc.contributor.other | School of Life Science and Technology, Institute of Science Tokyo, Midori-ku, Yokohama 2268501, Japan | |
| dc.contributor.other | Corresponding author.; School of Life Science and Technology, Institute of Science Tokyo, Midori-ku, Yokohama 2268501, Japan | |
| dc.date.accessioned | 2025-08-27T04:49:11Z | |
| dc.date.accessioned | 2025-10-08T09:07:34Z | |
| dc.date.available | 2025-10-08T09:07:34Z | |
| dc.date.issued | 01-10-2025 | |
| dc.identifier.uri | http://digilib.fisipol.ugm.ac.id/repo/handle/15717717/38900 | |
| dc.description.abstract | Background: 5-aminolevulinic acid-based photodynamic therapy (ALA–PDT) has emerged as a promising cancer treatment owing to its selectivity and minimal invasiveness. However, certain cancer types exhibit resistance to ALA–PDT, partly due to insufficient protoporphyrin Ⅸ (PpⅨ) accumulation. This study aimed to enhance PpⅨ accumulation by modulating iron metabolism. Methods: Two cancer cell lines were used in this study, TMK-1 and MCF-7. Divalent metal transporter 1 (DMT1) was knocked down genetically, and the iron chelator deferoxamine (DFO) was used to downregulate ferrochelatase (FECH). The cellular levels of PpⅨ, reactive oxygen species, Fe2+, and cell viability were determined to evaluate the efficacy of ALA–PDT. Results: Knocking down DMT1 increased PpⅨ accumulation in TMK-1 cells but did not contribute to it in MCF-7 cells. DFO enhanced PpⅨ accumulation in both cell lines, primarily by removing excess iron ions and inhibiting FECH. Conclusion: This study offers a novel strategy for improving the efficacy of ALA–PDT through the modulation of iron metabolism, achieved by targeting DMT1 and utilizing the iron chelator DFO. Additionally, DFO exhibited a dual mechanism, depending on cellular iron homeostasis. | |
| dc.language.iso | EN | |
| dc.publisher | Elsevier | |
| dc.subject.lcc | Medicine (General) | |
| dc.title | Improving 5-Aminolevulinic acid photodynamic therapy through the modulation of iron metabolism | |
| dc.type | Article | |
| dc.description.keywords | 5-aminolevulinic acid | |
| dc.description.keywords | Photodynamic therapy | |
| dc.description.keywords | Iron homeostasis | |
| dc.description.keywords | Divalent metal transporter 1 | |
| dc.description.keywords | Deferoxamine | |
| dc.description.doi | 10.1016/j.pdpdt.2025.104755 | |
| dc.title.journal | Photodiagnosis and Photodynamic Therapy | |
| dc.identifier.oai | oai:doaj.org/journal:5b0f5eb2d37d4870acec3e4858452377 | |
