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The Effects of Dietary Supplementation with 25-Hydroxyvitamin D3 on the Antioxidant Capacity and Inflammatory Responses of <i>Pelteobagrus fulvidraco</i>

dc.contributor.authorYi Liu
dc.contributor.authorJiang Xie
dc.contributor.authorQingchao Shi
dc.contributor.authorQuan Gong
dc.contributor.authorChuanjie Qin
dc.contributor.otherKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641000, China
dc.contributor.otherKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641000, China
dc.contributor.otherKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641000, China
dc.contributor.otherFishery Institute, Sichuan Academy of Agricultural Sciences, Chengdu 611731, China
dc.contributor.otherKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641000, China
dc.date.accessioned2025-08-27T14:12:21Z
dc.date.accessioned2025-10-08T09:11:29Z
dc.date.available2025-10-08T09:11:29Z
dc.date.issued01-08-2025
dc.identifier.urihttp://digilib.fisipol.ugm.ac.id/repo/handle/15717717/39289
dc.description.abstractBased on the limited hepatic hydroxylation efficiency of dietary VD3 in teleosts and the superior bioavailability of its metabolite, 25(OH)D3, this study investigated the regulatory mechanisms of dietary 25(OH)D3 supplementation in yellow catfish—an economically significant species lacking prior nutritional data on this metabolite. A total of 360 fish were divided into three groups—control (basal diet), VD3 (2500 IU/kg VD3), and 25(OH)D3 (2500 IU/kg 25(OH)D3)—and fed for 8 weeks. Compared to the control, both supplemented groups showed elevated superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), and transforming growth factor-β (<i>TGF-β</i>) activities, alongside reduced malondialdehyde (MDA), interleukin-1β (<i>IL-1β</i>), and tumor necrosis factor-α (<i>TNF-α</i>) levels. The 25(OH)D3 group exhibited higher T-AOC and CAT activities and lower <i>TNF-α</i> than the VD3 group. Metabolomic and transcriptomic analyses identified 65 differentially expressed metabolites (DEMs) and 3515 differentially expressed genes (DEGs). Enrichment analysis indicated that the DEMs (e.g., indole compounds, organic acids, aldosterone, L-kynurenine) and DEGs (<i>pgd</i>, <i>mthfr</i>, <i>nsdhl</i>, <i>nox5</i>, <i>prdx2</i>, <i>mpx</i>, <i>itih2</i>, <i>itih3</i>, <i>eprs1</i>) that were highly and significantly expressed in the 25(OH)D3 group were primarily associated with antioxidant defense and inflammatory responses. Dietary 25(OH)D3 was more effective than VD3 in promoting antioxidant capacity and modulating inflammation in yellow catfish.
dc.language.isoEN
dc.publisherMDPI AG
dc.subject.lccBiology (General)
dc.titleThe Effects of Dietary Supplementation with 25-Hydroxyvitamin D3 on the Antioxidant Capacity and Inflammatory Responses of <i>Pelteobagrus fulvidraco</i>
dc.typeArticle
dc.description.keywordsmetabolome
dc.description.keywordstranscriptome
dc.description.keywordsyellow catfish
dc.description.keywords25(OH)D3
dc.description.keywordsantioxidant
dc.description.keywordsinflammatory
dc.description.doi10.3390/biology14080967
dc.title.journalBiology
dc.identifier.e-issn2079-7737
dc.identifier.oaioai:doaj.org/journal:087374dcfe894ccf8b20f95e3f7877eb
dc.journal.infoVolume 14, Issue 8


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